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KMID : 0379120170450020114
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Korean Journal of Mycology
2017 Volume.45 No. 2 p.114 ~ p.120
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Development of Cleaved Amplified Polymorphic Sequence Markers for the Identification of Lentinula edodes Cultivars Sanmaru 1ho and Chunjang 3ho
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Moon Su-Yun
Lee Hwa-Yong
Kim Myung-Kil
Ka Kang-Hyeon
Ko Han-Kyu
Chung Jong-Wook
Koo Chang-Duck
Ryu Ho-Jin
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Abstract
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Lentinula edodes is an edible mushroom that is mainly cultivated in Asian countries. Recently, new cultivars of this mushroom have been developed in Korea; variety protection is very important, so the development of efficient molecular markers that can distinguish each variety is required. In this study, we developed cleaved amplified polymorphic sequence (CAPS) markers for the identification of cultivars (Sanmaru 1ho and Chunjang 3ho). These markers were developed from whole genomic sequencing data from monokaryon strain B17 and resequencing data from 10 dikaryon strains. A single nucleotide polymorphism changed in scaffold 9 POS 1630048 in Sanmaru 1ho(G→T), and in scaffold 13 POS 920681 in Chunjang 3ho (G→A). The restriction enzymes TspR I and Xho I distinguished Sanmaru 1ho and Chunjang 3ho, respectively, from other strains. Thus, we developed 2 CAPS markers for the identification of the cultivars Sanmaru 1ho and Chunjang 3ho.
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KEYWORD
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CAPS marker, Genome-wide, Lentinula edodes, Single nucleotide polymorphism
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